The research team discovered that when an enzyme and the special protein Cry3Aa were produced together in the same bacteria cell, the resulting crystals, which can be easily isolated, contained millions of copies of the enzyme trapped inside the pores. The entrapment of this enzyme inside the crystals was found to have a high enzyme loading, significantly improved stability, and more importantly, the ability to be utilised as a recyclable biodiesel catalyst. The findings, recently published in the Journal of the American Chemical Society, open up a new avenue for the enzymatic production of biodiesel from waste cooking oil.
Heater, B. S., Yang, Z., Lee, M. M., & Chan, M. K. (2020). In Vivo Enzyme Entrapment in a Protein Crystal. Journal of the American Chemical Society. DOI: https://doi.org/10.1021/jacs.9b13462